Table 1

Structure and sequences of c-myc antisense and control oligonucleotides

Methylphosphonodiester / phosphodiester chimeric oligodeoxynucleotides

5'F-MD685AS

5'–Fluos-C/T/G/C/T/G/T-C-G-T-T-G-A-G-A/G/G/G/T/A–3'

AS targeting 1147–1166

5'F-MD586iAS

5'–Fluos-A/T/G/G/G/A-G-A-G-T-T-G-C-T/G/T/C/G/T/C–3'

Inverted AS control

5'F-MD586S

5'–Fluos-T/A/C/C/C/T-C-T-C-A-A-C-G-A/C/A/G/C/A/G–3'

Sense 1147–1166 control

5'F-MD757AS

5'–Fluos-C/T/G/C/T/G/T/C-G-T-T-G-A/G/A/G/G/G/T/A–3'

AS targeting 1147–1166

CpG oligodeoxynucleotide 5-mer

CGTTG

5 '–Fluos-C-G-T-T-G-ProOH–3'

CpG motif present in 1147–1166 AS and 559–573 AS

Morpholino oligonucleotide analogues

M28AS

5'–Fluos-GGC AUC GUC GCG GGA GGC UGC UGG AGC G–3'

AS targeting from within Intron 1 to 563

M28NS

5'–Fluos-CAG GCC UUG UGG ACG GCG GUU CGC GAG G–3'

Nonsense control

M28S

5'–Fluos-CGC UCC AGC AGC CUC CCG CGA CGA UGC C–3'

Sense from within Intron 1 to 563 control


/ = Methylphosphonodiester link; - = phosphodiester link; Fluos = fluorescein label; -ProOH = 3-hydroxypropyl phosphate group Numbering refers to base positions in c-myc mRNA (HSMYC1, GenBank Accession Number V00568)

Tidd et al. BMC Molecular Biology 2001 2:13   doi:10.1186/1471-2199-2-13

Open Data