Comparison of the chimeric c-myc antisense 20-mer, 5'F-MD757AS (Table 1) with the CpG 5-mer motif CGTTG contained within its phosphodiester section for effects on c-myc mRNA, c-Myc protein and proliferation of KYO1 cells. Cells were loaded with oligodeoxynucleotide by streptolysin O permeabilization from an external concentration of 20 μM. (A) Cell proliferation over 24 h. (B) Levels of full length c-myc mRNA 30 min after oligodeoxynucleotide delivery. RNase H-generated mRNA fragments were visible on the Northern blot for cells treated with the 20-mer but not the 5-mer. (C) Relative levels of c-Myc protein. Values in all panels are the mean and standard deviation of 3 replicates.
Tidd et al. BMC Molecular Biology 2001 2:13 doi:10.1186/1471-2199-2-13