Effects of an RNase H-inactive morpholino antisense oligonucleotide on c-myc mRNA, c-Myc protein, and proliferation of KYO1 cells. The antisense, and control sense and nonsense oligonucleotides (Table 1) were delivered into cells by streptolysin O permeabilization from an external concentration of 20 μM. (A) Upper panels; Northern blots of mRNA at 4 and 24 h. Lower panels; Western blots of c-Myc protein at 4 and 24 h. The 47 kDa truncated protein containing the c-Myc epitope recognised by the antibody mix resulted from initiation of translation at an in-frame AUG 300 bases downstream of the c-Myc 2 AUG initiation codon . (B) Cell number relative to that at 2 h after loading cells with oligonucleotide. Values are the mean and standard deviation of 3 replicates.
Tidd et al. BMC Molecular Biology 2001 2:13 doi:10.1186/1471-2199-2-13