Table 4

Ranking of HKG stability by public algorithms
Ranking Ref finder Ge norm Norm finder Best keeper delta Ct
1 RPS13 (1.68) PPIA/RPS13 (0.600) RPL27 (0.244) PPIA (1.074) RPL27 (1.82)
2 PPIA (1.86) RPL27 (0.883) RPS13 (1.252) RPS13 (1.202) RPS13 (1.97)
3 RPL27 (1.86) RP2 (1.038) RP2 (1.276) b-Act (1.250) PPIA (2.01)
4 RP2 (3.94) b-Act (1.206) PPIA (1.387) RPL27 (1.286) RP2 (2.07)
5 b-Act (5.01) 18S (1.776) 18S (1.885) RP2 (1.520) 18S (2.51)
6 18S (5.48) B2M (2.084) B2M (2.198) 18S (2.367) b-Act (2.52)
7 B2M (6.74) GAPDH (2.343) b-Act (2.206) B2M (2.472) B2M (2.72)
8 GAPDH (8.00) GAPDH (2.844) GAPDH (3.161) GAPDH (3.12)

Each HKG (or pair of HKG) is ranked according to the indicated algorithms (respective stability scores indicated in brackets) and RefFinder assigned a final rank (geometric mean of stability scores for each gene). Lower values indicate more stable genes. Note that (i) PPIA and RPS13 rank at the top of 3 out of 5 algorithms; (ii) GAPDH and B2M are worst candidates; and (iii) beta-Actin ranks intermediate.

Dupasquier et al.

Dupasquier et al. BMC Molecular Biology 2014 15:9   doi:10.1186/1471-2199-15-9

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