Table 3

Evaluation by conventional statistical analysis of HKG levels and stability
Wilcoxon p value CtCV intraN (%) CtCV intraT (%) CtCV Tot (%)
18S 0.042* 15.94 29.15 24.52
β-Act 0.391 3.34 9.11 6.85
GAPDH 0.017* 7.71 13.13 12.72
B2M 0.042* 6.82 12.54 10.96
RP2 0.194 3.59 6.59 5.45
PPIA 0.626 3.58 6.66 5.36
RPL27 0.035* 3.97 7.06 6.26
RPS13 0.715 3.55 7.56 5.90

Statistical differences between Ct for tumoral versus normal tissue were assessed by non-parametric paired Wilcoxon test. Highest p values indicate best candidates; *(p < 0.05) indicates unsuitable HKG. Coefficients of variation (CtCV) are given for normal (intraN), tumoral (intraT) and all confounded (Tot) samples. Lower values reflect lesser dispersion and better stability.

Dupasquier et al.

Dupasquier et al. BMC Molecular Biology 2014 15:9   doi:10.1186/1471-2199-15-9

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