Additional file 2.
Effects of primer and choice of reference HKGs on the estimation of normalized YBX3 expression in ccRCC samples. Expression of YBX3 mRNA, measured with YBX3 + Ψ (open bars) and YBX3-Ψ (filled bars) primers, in each of the 16 tumors compared to adjacent healthy tissue (set at 1, red dotted line) after normalization to either beta-Actin (A), or PPIA & RPS13 (B). Values are means of 5 independent assays with standard deviations. Tumor values statistically different from their normal paired tissues are indicated by asterisks (*). Pairs 1 to 3 were those analyzed in our previous report .
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Dupasquier et al. BMC Molecular Biology 2014 15:9 doi:10.1186/1471-2199-15-9