Effects of primer choice of reference HKG on the estimation of normalized YBX3 expression in ccRCC samples. Circles represent expression ratios distribution of YBX3 mRNA (YBX3-Ψ, filled circles; YBX3 + Ψ, open circles) in each of the 16 tumors compared to its normal paired tissue (set at 1, dot line) after normalization by beta-Actin (A) versus PPIA + RPS13 (B). Values are means of 5 independent assays (for the sake of clarity, standard deviations and statistical significance for each sample are not shown, but are provided in the histograms of Additional file 2). Note that pairs 1 to 3 were analyzed in the previous report . Lines are medians with indicated values. After normalization by beta-Actin, YBX3 appears significantly overexpressed in tumors taken as a whole (A, **p < 0.01, *p < 0.05, Wilcoxon test) while it is not the case when normalized by PPIA and RPS13 (B, NS: not significant, Wilcoxon test).
Dupasquier et al. BMC Molecular Biology 2014 15:9 doi:10.1186/1471-2199-15-9