|Effect of the AAV ITR sequence on gene targeting and random integration in yeast|
|Plasmid||Gene targeting||Random integration||GT/RI|
|ITR-lys2URA3lys2-ITR||31.0 ± 11.3 (7025)||0.62 ± 0.37 (487)||50.2|
|lys2URA3lys2||35.4 ± 10.4 (6960)||1.82 ± 0.41 (3420)*||19.4|
Yeast was transformed with PvuII- or XbaI-restricted pAAVLUL as reported in Materials and Methods. Gene targeting and random integration events are reported as number of URA3+lys2-colonies /103 transformants per μg of plasmid DNA and URA3+LYS2+colonies /103 transformants per μg of plasmid DNA, respectively. The number of transformants per μg of DNA was determined by transformation with 1 μg of episomal plasmid DNA. Results are the mean of four independent experiments ± standard deviation. The number of total colonies is indicated in parentheses. Statistical analysis was performed using Student’s t- test. *p = 0.0048 vsITR-lys2URA3lys2-ITR.
Galli and Cervelli
Galli and Cervelli BMC Molecular Biology 2014 15:5 doi:10.1186/1471-2199-15-5