Table 1

Effect of the AAV ITR sequence on gene targeting and random integration in yeast
Plasmid Gene targeting Random integration GT/RI
ITR-lys2URA3lys2-ITR 31.0 ± 11.3 (7025) 0.62 ± 0.37 (487) 50.2
lys2URA3lys2 35.4 ± 10.4 (6960) 1.82 ± 0.41 (3420)* 19.4

Yeast was transformed with PvuII- or XbaI-restricted pAAVLUL as reported in Materials and Methods. Gene targeting and random integration events are reported as number of URA3+lys2-colonies /103 transformants per μg of plasmid DNA and URA3+LYS2+colonies /103 transformants per μg of plasmid DNA, respectively. The number of transformants per μg of DNA was determined by transformation with 1 μg of episomal plasmid DNA. Results are the mean of four independent experiments ± standard deviation. The number of total colonies is indicated in parentheses. Statistical analysis was performed using Student’s t- test. *p = 0.0048 vsITR-lys2URA3lys2-ITR.

Galli and Cervelli

Galli and Cervelli BMC Molecular Biology 2014 15:5   doi:10.1186/1471-2199-15-5

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