Molecular analysis of random integration clones derived from transformation with no-ITRs-containing fragment. Southern blot analysis of genomic DNA from fourteen URA3+LYS2+ yeast clones derived from transformation of RSY12 yeast strain with pAAVLUL digested with XbaI. Digestion with XbaI produces a fragment without ITR as described in Figure 1B. Genomic DNA was digested with AseI that does not cut in the sequence between the ITRs. The numbers above the filters indicate the clones. Bands were detected using the URA3 probe.
Galli and Cervelli BMC Molecular Biology 2014 15:5 doi:10.1186/1471-2199-15-5