Molecular analysis of random integration clones derived from transformation with ITRs carrying fragment. Southern blot analysis of genomic DNA isolated from URA3+LYS2+ yeast transformant clones. These clones were obtained by transforming RSY12 yeast strain with the ITRs-containing fragment obtained by the digestion of pAAVLUL vector with PvuII. We analyzed genomic DNA digested with AseI of eleven different clones. The numbers above the filters indicate the clones. Bands were detected using the URA3 probe and the ITR probe as indicated.
Galli and Cervelli BMC Molecular Biology 2014 15:5 doi:10.1186/1471-2199-15-5