Mutational analysis of Pax-like cis-elements in the RPP: Effect on Otx5-LNrl activation. (A) Diagram indicating the location of the Ret1, BAT1, NRE, Ret4 and TATA box (TA) elements and the mutations (TT to GG) constructed in these sequences. The transcription start site is marked by +1. (B) Cells were transiently transfected with a plasmid containing a wild type promoter (XOP(−503/+41)) or one of the mutants (m1-m10) in the absence (No TF) or presence of Otx5-LNrl. Activities in cell lysates are presented as mean ± S.E.M (n = 6-8). Promoter basal activity (RLU) is shown in parentheses.
Reks et al. BMC Molecular Biology 2014 15:4 doi:10.1186/1471-2199-15-4