Email updates

Keep up to date with the latest news and content from BMC Molecular Biology and BioMed Central.

Open Access Highly Accessed Open Badges Correction

Correction: Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family

Agnieszka Zylicz-Stachula1, Janusz M Bujnicki2 and Piotr M Skowron1*

Author Affiliations

1 Division of Molecular Biotechnology, Department of Chemistry, Institute for Environmental and Human Health Protection, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland

2 Laboratory of Bioinformatics and Protein Engineering, International Institute of Molecular and Cell Biology in Warsaw, Ks. Trojdena 4, 02–109 Warsaw, and Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 69, Poznan, Poland

For all author emails, please log on.

BMC Molecular Biology 2014, 15:16  doi:10.1186/1471-2199-15-16

Published: 5 August 2014

First paragraph (this article has no abstract)

The TspGWI restriction endonuclease, which originates from thermophilic Thermus sp. GW, was cloned previously [1] in Escherichia coli (E. coli) using a method employing aa sequencing of proteolytic fragments N-termini, followed by a combination of sequencing of degenerated, inverse and standard PCR products and clones containing the tspGWIRM gene. A combination of these methods yielded a sequence of 3688 bp, comprising an entire TspGWI Open Reading Frame (ORF: 3291 bp) and flanking sequences [GenBank: EF095488, ABO26710]. However, more recent resequencing of the tspGWIRM gene with the use of genomic Thermus sp. GW DNA as a template in PCR, obtained with primers external to ORF, has revealed a sequencing error. This resulted in a frameshift of 233 aa, starting at aa 740 and returning to the original ORF at aa 973. The frameshift was located within the 3′-terminal portion of the gene, coding for a Target Recognition Domain (TRD). The nt sequence of the tspGWIRM gene was corrected at 2217 bp (C insertion) and further downstream at 2915 nt (C deletion), restoring the ORF (Additional file one (Additional file 1 here) and Figure five (Figure 1 here) corrected).