Open Access Research article

Pseudo attP sites in favor of transgene integration and expression in cultured porcine cells identified by streptomyces phage phiC31 integrase

Yanzhen Bi1*, Ximei Liu1, Long Zhang12, Changwei Shao3, Zhuo Ma14, Zaidong Hua1, Liping Zhang1, Li Li1, Wenjun Hua1, Hongwei Xiao1, Qingxin Wei1 and Xinmin Zheng1*

Author Affiliations

1 Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Science and Veterinary Medicine, Hubei Academy of Agricultural Science, Wuhan 430064, China

2 College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China

3 College of Life Science, Wuhan University, Wuhan 430072, China

4 College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

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BMC Molecular Biology 2013, 14:20  doi:10.1186/1471-2199-14-20

Published: 8 September 2013

Additional files

Additional file 1: Figure S1:

ABI-REC of pig pseudo attP sites into pBCPB+ plasmid. Fused products were indicated by arrow. M is molecular DNA ladder. 5113, 5156, 1015 and 2015 represents four pig pseudo attP sites cloned by ABI-REC.

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Additional file 2: Figure S2:

p’BCPB+ plasmid restriction analysis. Recombinant plasmids were analyzed by restriction digestion. M is molecular DNA ladder. 5113, 5156, 1015 and 2015 stands for the four recombinant plasmids derived from backbone pBCPB+. For each plasmid, the first lane shows undigested DNA and the second lane shows the restricted DNA.

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Additional file 3: Figure S3:

Partial sequences of four p’BCPB+ plasmids were shown. Native restriction site is underlined. Cloned pig pseudo attP site is shown in red, where the identified pseudo attP site is shown in bold. pBCPB+ plasmid sequence is shown in black. Recombination crossover is shown in bold and italics.

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