Additional file 3.
Figure S3. in situ PLA reveals interaction between DNA-PK and histone H3 in cells treated with NCS and expressing CK2. A. Association between DNA-PKcs and histone H3 was investigated by in situ PLA in M059K cells treated as indicated in the figure and exposed to 0.5 μg/ml NCS for 1 hour. The molecular interaction is indicated by the presence of distinct red fluorescent spots in the cell nuclei. Control indicates cells treated with si-Scr and NCS and stained with the secondary antibodies after fixation. B. Quantification of the number of positive signals/cell was performed by computer-assisted image analysis. Mean values +/- SD from three independent experiments are shown. *P < 0.0001 denotes statistically significant difference between cell populations treated with si-Scr and siRNAs against CK2α', respectively.
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Olsen et al. BMC Molecular Biology 2012 13:7 doi:10.1186/1471-2199-13-7