Cells overexpressing CK2 exhibit a prompt response to induction of DNA double-strand break. A. M059K cells transiently overexpressing CK2α'-DsRed or DsRed-fluorescent protein were incubated with 0.5 μg/ml NCS for the indicated times. Subsequently, cells were fixed and labeled with anti-γ-H2AX antibody. Cell nuclei were revealed by DAPI staining. Arrows: cells overexpressing CK2α'-DsRed are more efficient in DNA damage repair as compared to cells that do not overexpress the kinase. One representative experiment is shown. B. Average γ-H2AX foci number per red fluorescent cell as described in A. Mean values +/- SD from three independent experiments are shown. More than 100 cells have been analyzed per experiment. *P < 0.001 denotes statistically significant difference. NS, not significant.
Olsen et al. BMC Molecular Biology 2012 13:7 doi:10.1186/1471-2199-13-7