Additional file 4.
Figure S3. The activity of siRNAs targeting SNP sites in the SCA1 model. (A) The target sites and the nucleotide sequences of siRNAs directed at the rs179990 SNP in the ATXN1 gene. The nucleotides in the antisense strand that directly interact at the SNP site are marked in bold. The mismatches introduced into the siRNA duplex are in gray boxes. RNA is in lowercase; DNA is in uppercase. (B) The RT-PCR analysis of ATXN1 expression at the transcript level in the SCA1 fibroblasts (GM06927) at 72 h after transfection with 50 nM siRNAs. (C) The RT-PCR analysis of ATXN1 transcript level in the SCA1 fibroblasts at 72 h after transfection with 2, 10, 50 or 200 nM siRNA G10. C - the reference bar indicates the ATXN1 expression level in the cells transfected with the control siRNA. In the graphs, the signal intensities were normalized to the GAPDH mRNA level and compared using a one-sample t-test. The error bars represent standard deviations. The P-value is indicated by asterisks (* p < 0.001; ** 0.001 < p < 0.05).
Format: TIFF Size: 582KB Download file
Fiszer et al. BMC Molecular Biology 2012 13:6 doi:10.1186/1471-2199-13-6