Orientation-dependent silencing by ARSs. ARS305, ARS605, ARS317 and ARS319 were cloned in URA3-ACS-B1-tel (upper part) and URA3-B1-ACS-tel (lower part) orientation and inserted in the VIIL telomere. Levels of URA3 silencing were assessed as % FOAR cells and plotted. Data is from Table 2. Statistical significance (p values) for the difference between the control construct (no ARS, middle of the graph) and the ARSs in URA3-B1-ACS-tel orientation are shown next to each bar. The p values for the constructs in URA3-ACS-B1-tel orientation are significantly lower than 0.05 and are not shown.
Chisamore-Robert et al. BMC Molecular Biology 2012 13:34 doi:10.1186/1471-2199-13-34