|Viability of single and double mutant strains inYHR087WandCDC33under hygromycin 10 μg/mL|
|BY4741||95,33 ± 8,08|
|Δyhr087w||73,67 ± 0,58|
|cdc33ts||41,71 ± 8,33|
|Δyhr087w cdc33ts||27,34 ± 3,45|
Dilutions from cultures in exponential phase of growth were plated on YPD or YPD + hygromycin 10 μg/mL plates and the colony forming units appeared 48-72 hours later were counted. Viability was determined as the percentage between the CFU in hygromycin plates and in YPD plates. Application of the t-test to the data obtained for cdc33ts and Δyhr087w cdc33ts mutant strains gave a P value (calculated for one tail using Microsoft Office Excel) of 0.0025.
Gomar-Alba et al.
Gomar-Alba et al. BMC Molecular Biology 2012 13:19 doi:10.1186/1471-2199-13-19