Isolation of VDR-protein complex from human keratinocyte cell lysate. (A) Overview of the experimental procedure of the pull-down assay. (B) Western blot analysis with anti-PIM1 and anti-VDR antibody. PIM-1 and VDR were observed only in the lysate captured by the DRIP-2 peptide, while in the control lysate without peptide only unspecific lanes appeared. The expected sizes were for VDR about 48 kD, for PIM-1 about 34 kD and for the putative VDR/PIM-1 complex (unspecific band) respectively about 82 kD.
Maier et al. BMC Molecular Biology 2012 13:18 doi:10.1186/1471-2199-13-18