Additional file 3.

mRNA expression of inducible genes in CD4+ T cells. Total RNA was prepared from primary CD4+ T cells, either unstimulated or stimulated with PMA/I for the indicated times. A. Expression of Il2 and Csf2 in primary CD4+ T cells isolated from both age groups of mice was normalized to the Ubc housekeeping gene. B & C. Expression of Il2 and Csf2 in primary CD4+ T cells isolated from 2 week (B) and 16 week (C) old C57BL/6 mice are presented as fold change relative to unstimulated cells in addition to initial Ubc normalization as mentioned in A. Normalized gene expression is shown on a log scale. All experiments were repeated at least 3 times

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Li et al. BMC Molecular Biology 2012 13:16   doi:10.1186/1471-2199-13-16