Additional file 2.
mRNA expression of inducible genes in CD4+ T cells. Total RNA was prepared from non-differentiated naive T cells, and differentiated Th1, Th2, Th17, nTreg and iTreg cells. After reverse transcription, quantitative PCR analysis was performed on cDNA with primers designed to detect inducible genes. Expression of Ifng, Il4, Il17 and Foxp3 in all the undifferentiated and differentiated T cells were normalized to the Ubc housekeeping gene. The data are shown as the mean and SEM of at least three independent experiments
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Li et al. BMC Molecular Biology 2012 13:16 doi:10.1186/1471-2199-13-16