Matched samples yield fewer dissimilar regions of chromatin. Histogram illustrating the frequency of Pearson correlations (r) for comparisons between MNase-seq datasets for all tiling 1000 bp windows across the S. cerevisiae genome at 10 bp resolution. Comparisons were made between biological replicates, including our matched digest experiment (Strain #1 R1 & R2; red dotted line) and other published wild-type replicates (see Additional file 2: Table S1 for experimental details and references). Additionally, a comparison of matched wild-type and tup1Δ MNase-seq data is also illustrated (black). Results demonstrate how our matched preparation consistently produces MNase-seq datasets that are more closely correlated than other unstandardized preparations.
Rizzo et al. BMC Molecular Biology 2012 13:15 doi:10.1186/1471-2199-13-15