Open Access Research article

Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities

Agnieszka Zylicz-Stachula1, Olga Zolnierkiewicz2, Arvydas Lubys3, Danute Ramanauskaite3, Goda Mitkaite3, Janusz M Bujnicki4 and Piotr M Skowron2*

Author Affiliations

1 Division of Theoretical Physical Chemistry, Department of Chemistry, University of Gdansk, Sobieskiego 18, 80-952 Gdansk, Poland

2 Division of Environmental Molecular Biotechnology, Department of Chemistry, University of Gdansk, Sobieskiego 18, 80-952 Gdansk, Poland

3 Thermo Fisher Scientific, V.A.Graiciuno 8, LT-02241 Vilnius, Lithuania

4 Laboratory of Bioinformatics and Protein Engineering, International Institute of Molecular and Cell Biology in Warsaw, Ks. Trojdena 4, 02-109 Warsaw, and Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 69, Poznan, Poland

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BMC Molecular Biology 2012, 13:13  doi:10.1186/1471-2199-13-13

Published: 10 April 2012

Additional files

Additional file 1:

Scheme of sequencing and cloning of the tspDTRM gene.

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Additional file 2:

DNA and amino acid sequence of the tspDTIRM gene and its flanking regions. The predicted amino acid sequence of the 126.9 kDa TspDTI protein is indicated in capital letters. The DNA sequences of the flanking regions are indicated in italics. The internal amino acid sequences of the TspDTI enzyme, determined by chemical analysis of proteolytic fragments, are underlined. The ATG start codon is in bold. The TGA stop codon is shown in red. The potential TspDTI Ribosome Binding Sites (RBS) are boxed and in italics. The crucial amino acids of the catalytic centres are dark red, bold and underlined.

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Additional file 3:

DNA and amino acid sequence of the tsoIRM gene and its flanking regions. The predicted amino acid sequence of the 1116 amino acids/126.5 kDa TsoI protein as based on cloned tsoIRM gene is shown in capital letters (manuscript in preparation). The crucial amino acids of the catalytic centres are dark red, bold and underlined.

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