Effects of AML1-ETO on the MTG16 and MTGR1 promoter reporter. Erythroid HEL cells were co-transfected with 15 μg MTG16 -668 to -57 bp promoter plasmid (-668) or MTGR1 -499 to +52 bp promoter plasmid (-499) and 0 to 1 μg of AML1- ETO plasmid. pGL3/Basic (Basic). pGL3/SV40 (SV40). The luciferase activity is normalized against MTG16 -668 to -57 bp or MTGR1 -499 to +52 bp promoter. Both promoters are repressed in a dose-dependent manner by AML-ETO. Western blotting shows exogeneous AML1-ETO expression detected with anti-MTG. The experiments were repeated three times with similar results.
Ajore et al. BMC Molecular Biology 2012 13:11 doi:10.1186/1471-2199-13-11