Figure 11.

Reporter gene analysis of the MTGR1 promoter. Functional analysis by sequential 5'-deletion. The 5'deleted MTGR1 reporter constructs -989 to +52 (-989), -656 to +52 (-656), -499 to +52 (-499), -308 to +52 (-302), -257 to +52 (-257), -207 to +52 (-257), and -145 to +52 (-145) were examined after expression in HEL cells. The transcriptional activity is retained by the -499 to +52 bp region indicating the location of the proximal MTGR1 promoter. The pGL3/basic and pGL3/SV40-promoter are used as negative and positive control, respectively in (A) and (B). The luciferase activity was normalized against pGL3/SV40-promoter activity and is shown for 3 to 5 separate transfections; bars represent the mean and the error bars show SEM.

Ajore et al. BMC Molecular Biology 2012 13:11   doi:10.1186/1471-2199-13-11
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