Inhibition of the MTG16 promoter activity through inhibition of GATA-1 by HERP2. TF-1, HEL, UT-7, or MEG-01 cells were co-transfected with 10 μg MTG16 -668 to -57 bp promoter and the HERP2 plasmid construct. White bars show MTG16 -668 to -57 bp promoter activity without HERP2. Black bars show activity after co-transfection with HERP2, relative to the luciferase activity of the MTG16 -668 to -57 bp promoter in the absence of HERP2 (white bars). All cell lines examined demonstrated repression of GATA-1 driven MTG16 luciferase promoter. Renilla was not affetected by HERP2. The experiments were performed in triplicate. Bars represent the mean and the error bars show SEM.
Ajore et al. BMC Molecular Biology 2012 13:11 doi:10.1186/1471-2199-13-11