Overexpression of SENP1 raises PR-B sensitivity to hormone. HeLa cells were transfected with 50 ng of the PR-B (A) or PR-B K388R (C) expression vectors, a PRE2-luc, and Renilla control plasmid in the presence or absence of 100 ng of SENP1 expression vector and treated with ethanol or various concentrations of R5020 for 24 h. The average was plotted as a percentage of the maximal induction by 10 nM R5020 above no hormone levels. Curve fitting was performed by Prism Graph as described under "Experimental Procedures". The S.D. of triplicate values is indicated by the error bars. The corresponding relative luciferase activities were plotted for PR-B (B) and PR-B K388R (D). Statistical significance was computed by unpaired student's t test. *p < 0.05.
Abdel-Hafiz and Horwitz BMC Molecular Biology 2012 13:10 doi:10.1186/1471-2199-13-10