Table 2

Yeast strains and plasmids used in this study.

Strain

Relevant genotype

Source or reference


Isogenic to W303


EYL682

MATa mpk1Δ::TRP1 ura3-1 trp1-1 leu2-3,112 ade2-1 ade3-1 his3 can1-100 lys2 (Gal+)

Elion lab collection

EYL780

MATa bar1Δ far1Δ ste11-4 ste12Δ::URA3 trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2

Elion lab collection

EY957

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+)

Elion lab collection

EY1298

MATa bar1Δ far1Δ ste11-4 trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2

Elion lab collection

EYL4661

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ste11Δ::Hygro

Elion lab collection

EYL5328

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) pbs2Δ::Hygro

This study

EYL5329

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) kss1Δ::HIS3 fus3Δ::Hygro pbs2Δ::Kan

This study

EYL5330

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ste12Δ::Hygro

This study

EYL5331

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) bni1Δ::Hygro

This study

EYL5332

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ste7Δ::Hygro pbs2Δ::Kan

This study

EYL5333

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) cmk2Δ::Hygro

This study

EYL5334

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ptk2Δ::Hygro

This study

EYL5335

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) cnb1Δ::Hygro

This study

EYL5336

MATa bar1Δ far1Δ trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2 pbs2Δ::Hygro

This study

EYL5337

MATa bar1Δ far1Δ trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2 cnb1Δ::Hygro

This study

EYL5338

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) kss1Δ::HIS3 fus3Δ::Hygro

This study

EYL5379

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ptk2Δ::Hygro cmk2Δ::Kan

This study

EYL5380

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) cmk2-tag(3-GFP)Kan

This study

EYL5382

MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) crz1Δ::Kan

This study

S288c deletion strains in Figure 1 are isogenic to S288c BY4741

[46]

BY4741

MATa his3Δ1 ura3Δ0 leu2Δ0 met15-0

[47]

RG2468

ste4Δ::KANr leu2 ura3 his3 met15

Research Genetics

RG4038

ste5Δ::KANr leu2 ura3 his3 met15

Research Genetics

RG3439

ste50Δ::KANr leu2 ura3 his3 met15

Research Genetics

RG5271

ste11Δ::KANr leu2 ura3 his3 met15

Research Genetics

RG6981

kss1Δ::KANr leu2 ura3 his3 met15

Research Genetics

JAY408

fus3Δ::KANr ste5Δ::KANr

[25]

Isogenic to ∑1278b

L5528

ura3-52, his3::hisG

[48]

L5554

ste5::LEU2, ura3-52, leu2::hisG

[48]

Plasmids

pYGU-11ΔN

pGAL1-STE11ΔN LEU2 CEN

[49]

GAL1 vector

pGAL1, LEU2 CEN

Elion lab collection

pYEE133

pGAL1-STE12 TRP1 CEN

Elion lab collection

GAL1 vector

pGAL1, TRP1 CEN

Elion lab collection

pOO1

pCMK2-lacZ, URA3, 2 μ

[19]

EBL95

pDL1460 pFUS1::ubiY-lacZ, URA3, 2 μ

Elion lab collection

[50]

EBL183

PMC1-lacZ, URA3, 2 μ

[26]

EBL185

pDM5 FKS2 (-968-+6)-CYC1-lacZ, URA3, 2 μ

[26]

EBL201

FKS2 (-928-+1)-CYC1-lacZ, URA3, 2 μ

[14]

EBL200

FKS2 (-706-+1)-CYC1-lacZ, URA3, 2 μ

[14]

EBL1258

FKS2 (-540--375)-CYC1-lacZ, URA3, 2 μ

[15]

EBL513

YKL161c (MLP1)-lacZ, URA3, 2 μ

Elion lab collection

EBL514

YIL117c (PRM5)-lacZ, URA3, 2 μ

[51]

EBL517

pLGΔ-178 pCYC1-lacZ, URA3, 2 μ

[52]


Wang et al. BMC Molecular Biology 2011 12:51   doi:10.1186/1471-2199-12-51

Open Data