Research article
A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum
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* Corresponding author: Dieter Oesterhelt oesterhe@biochem.mpg.de
1 Max-Planck Institute of Biochemistry, Department of Membrane Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
2 Institut für Klinische Chemie, Klinikum Schwabing, Am Kölner Platz 1, 80804 Munich, Germany
3 Max-Planck Institute of Molecular Plant Physiology, Department 2, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany
4 School of Biomedical Sciences, Charles Sturt University, 2678 NSW, Australia
BMC Molecular Biology 2011, 12:42 doi:10.1186/1471-2199-12-42
Published: 19 September 2011Additional files
Additional file 1:
Genetic map of the genes and open reading frames in the bop gene region. Numbers indicate chromosomal coordinates of the genes and ORFs.
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Additional file 2:
Comparison of Brz, brz and Brb, brb sequences from strains of Hbt. salinarum R1 [21], S9 [16]and Hrb. utahensis. Red letters correspond to mutations.
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Additional file 3:
Comparison of Brb and brb sequences from Hbt. salinarum wild-type strain R1 and S9, brbM1, brzS9brbM2 mutants. Red letters correspond to mutations.
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Additional file 4:
Operon organization of the bp4 and OE2447F genes of Hbt. salinarum R1. The protein sequences are given below to nucleotide sequences. The arrow above the nucleotide line indicates the neighboring genes. Underlined letters correspond to translation start codons and boxed letters are translation stop codons.
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Additional file 5:
Operon organization of the OE3131F and bp2 genes of Hbt. salinarum R1. The protein sequences are given below to nucleotide sequences. The arrow above the nucleotide line indicates the neighboring genes. Underlined letters correspond to translation start codons and boxed letters are translation stop codons.
Format: PDF Size: 152KB Download file
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Additional file 6:
Organization of the HQ1083B and bp genes of Hqr. Walsbyi. The protein sequences are given below to nucleotide sequences. The arrow above the nucleotide line indicates the neighboring genes. Underlined letters correspond to translation start codons and boxed letters are translation stop codons.
Format: PDF Size: 218KB Download file
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Additional file 7:
Organization of the bop related genes cluster of Hrb. utahensis. The protein sequences of the bp gene are given below to nucleotide sequences. The arrow above the nucleotide line indicates the neighboring genes. Underlined letters correspond to translation start codons and boxed letters are translation stop codons.
Format: PDF Size: 186KB Download file
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Additional file 8:
DNA microarray analysis of differentially expressed genes from Hbt. salinarum R1 and R1M1 cells grown in the presence of novobiocin.
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Additional file 9:
Primers used in this work.
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