Strand-specific RT-PCR targeting P. gingivalis W83 genes. Antisense sequences targeted by RT-PCR: PG0279, PG0933, PG1069, PG0559, and PG0775 (marked A1-5, respectively). A3L and A5L were longer PCR products from PG1069 and PG0775. C1, PG1159 and C2, PG1144 were sense sequences targeted by RT-PCR. Columns from left to right: 1, RT-PCR amplicons on RNA duplicates for the targeted antisense and sense sequences; 2, PCR of the targeted antisense and sense sequences on gDNA; 3, RT-PCR of the targeted antisense sequences without reverse transcriptase; 4, RT-PCR amplicons on RNA duplicates targeting the mutB gene; 5, RT-PCR of mutB without reverse transcriptase.
Yu et al. BMC Molecular Biology 2011 12:3 doi:10.1186/1471-2199-12-3