Comparison of RNA- and cDNA-based transcriptome profiles. Normalized log2 probe signal intensities (y axis) from a 100-kbp region of P. gingivalis genome were plotted on the genomic sequence coordinate (x axis) based on the positions of the probe sequences in the genome. The genomic positions of open reading frames (ORFs) within this region from both forward (orange boxes) and reverse (turquoise boxes) strands are shown in the middle of the figure. The gray dashed line in each of the four profiles represents the baseline. The regions highlighted in gray contain potential artifactual cDNA signals from the antisense strand of the corresponding ORF. The black bars labeled "A5/A5L PCR product" depict the positions and sizes of the expected strand-specific RT-PCR results referred to in Figure 3.
Yu et al. BMC Molecular Biology 2011 12:3 doi:10.1186/1471-2199-12-3