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Open Access Highly Accessed Methodology article

Strand-specific transcriptome profiling with directly labeled RNA on genomic tiling microarrays

Wen-Han Yu12, Hedda Høvik3, Ingar Olsen3 and Tsute Chen1*

Author Affiliations

1 Department of Molecular Genetics, The Forsyth Institute, Cambridge, MA, USA

2 Bioinformatics Graduate Program, Boston University, Boston, MA, USA

3 Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway

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BMC Molecular Biology 2011, 12:3  doi:10.1186/1471-2199-12-3

Published: 14 January 2011

Additional files

Additional file 1:

The additional file includes a table listing the primer sequences used in this work and four figures showing additional results including the efficiency of RNA labeling, the effects of blocking reagent on the background signals, and the effects of actinomycin D added in the reverse transcription reaction.

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