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Open Access Highly Accessed Research article

Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

Kang Zhou1, Lihan Zhou12, Qing 'En Lim2, Ruiyang Zou1, Gregory Stephanopoulos13 and Heng-Phon Too12*

Author Affiliations

1 Chemical and Pharmaceutical Engineering, Singapore-MIT Alliance, 4 Engineering Drive 3, Singapore

2 Department of Biochemistry, National University of Singapore, 8 Medical Drive, Singapore

3 Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, The USA

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BMC Molecular Biology 2011, 12:18  doi:10.1186/1471-2199-12-18

Published: 23 April 2011

Additional files

Additional file 1:

supplementary figures S1-S6 and supplementary tables S1-S3. supplementary figures and tables for the manuscript.

Format: PPTX Size: 1.7MB Download file

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Additional file 2:

list of overexpressed genes in the selected microarray studies. the file contains the names of overexpressed genes in the selected microarray studies and their functional categories.

Format: XLSX Size: 11KB Download file

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Additional file 3:

geometric mean calculation table. an excel table to facilitate calculation of geometric mean of multiple reference genes.

Format: XLSX Size: 10KB Download file

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Additional file 4:

MIQE checklist. the checklist to ensure that the study was done according to the MIQE guidelines.

Format: XLS Size: 36KB Download file

This file can be viewed with: Microsoft Excel Viewer

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