Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR
1 Chemical and Pharmaceutical Engineering, Singapore-MIT Alliance, 4 Engineering Drive 3, Singapore
2 Department of Biochemistry, National University of Singapore, 8 Medical Drive, Singapore
3 Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, The USA
BMC Molecular Biology 2011, 12:18 doi:10.1186/1471-2199-12-18Published: 23 April 2011
Additional file 1:
supplementary figures S1-S6 and supplementary tables S1-S3. supplementary figures and tables for the manuscript.
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Additional file 2:
list of overexpressed genes in the selected microarray studies. the file contains the names of overexpressed genes in the selected microarray studies and their functional categories.
Format: XLSX Size: 11KB Download file
Additional file 3:
geometric mean calculation table. an excel table to facilitate calculation of geometric mean of multiple reference genes.
Format: XLSX Size: 10KB Download file