Table 7

Kinetic parameters for ATP and dATP hydrolysis catalyzed by DmuRecA.

Substrate

DNA

T (°C)

KM (mM)

kcat (s-1)

kcat/KM (s-1 M-1)

na


ATP

ssDNA

37

0.31 ± 0.009

0.13 ± 0.004

4.3 ± 0.18 × 102

1.1

ssDNA and dsDNA

37

0.28 ± 0.009

0.067 ± 0.0021

2.4 ± 0.08 × 102

1.5


dATP

ssDNA

37

0.28 ± 0.007

0.14 ± 0.004

5.0 ± 0.25 × 102

1.0

ssDNA and dsDNA

37

0.22 ± 0.007

0.073 ± 0.0023

3.4 ± 0.05 × 102

1.3


a Hill coefficient

Wanarska et al. BMC Molecular Biology 2011 12:17   doi:10.1186/1471-2199-12-17

Open Data