Table 6

Kinetic parameters for ATP and dATP hydrolysis catalyzed by DgeRecA.

Substrate

DNA

T (°C)

KM (mM)

kcat (s-1)

kcat/KM (s-1 M-1)

na


ATP

ssDNA

37

0.56 ± 0.018

0.15 ± 0.004

2.7 ± 0.12 × 102

2.9

ssDNA and dsDNA

37

0.13 ± 0.006

0.13 ± 0.006

1.0 ± 0.04 × 103

1.4


dATP

ssDNA

37

0.62 ± 0.023

0.21 ± 0.007

3.3 ± 0.10 × 102

1.2

ssDNA and dsDNA

37

0.36 ± 0.016

0.15 ± 0.007

4.2 ± 0.16 × 102

1.0


a Hill coefficient

Wanarska et al. BMC Molecular Biology 2011 12:17   doi:10.1186/1471-2199-12-17

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