Table 5

Effect of temperature on the rate of ssDNA-dependent ATP and dATP hydrolysis catalyzed by DgeRecA and DmuRecA.

Protein

Substrate

Rate of hydrolysis (μM/min)

32°C

37°C

42°C

45°C

50°C


DgeRecA

ATP

19.8 ± 0.26

26.8 ± 0.40

31.2 ± 0.56

34.1 ± 0.78

26.6 ± 0.43

dATP

10.3 ± 0.18

31.6 ± 0.63

53.7 ± 1.18

65.7 ± 1.71

56.5 ± 1.30


DmuRecA

ATP

15.6 ± 0.23

34.4 ± 0.58

55.2 ± 1.16

47.6 ± 1.20

34.5 ± 0.62

dATP

24.2 ± 0.34

47.0 ± 0.71

70.3 ± 1.76

59.9 ± 1.38

43.9 ± 0.88


Reaction conditions: 2.27 mM ATP or dATP.

Wanarska et al. BMC Molecular Biology 2011 12:17   doi:10.1186/1471-2199-12-17

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