Table 4

Effect of DNA on the rate of ATP and dATP hydrolysis catalyzed by DmuRecA.

Substrate

Rate of hydrolysis (μM/min)

absence of DNA

dsDNA

ssDNA

ssDNA and dsDNAa


ATP

not detected

not detected

34.4 ± 0.58

21.2 ± 0.44

dATP

not detected

not detected

47.0 ± 0.71

24.6 ± 0.49


Reactions conditions: 37°C, 2.27 mM ATP or dATP.

a DNA strand exchange conditions

Wanarska et al. BMC Molecular Biology 2011 12:17   doi:10.1186/1471-2199-12-17

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