Observed ranges of Cq values of the 21 preselected reference gene candidates during phorbol ester-induced monocyte-to-macrophage differentiation of THP-1 cells. Cq values were recorded for six days of differentiation as described in the Methods section. Boxes indicate the 25 and 75 percentile, means are indicated by squares, and whiskers represent maximum and minimum data points.
Maeß et al. BMC Molecular Biology 2010 11:90 doi:10.1186/1471-2199-11-90