Table 6

Results of the gene expression analysis with REST for aTub, GAPDH and MMP with different normalization strategies

Normalized against

Target gene

x-fold change in gene expression*

SE range

P


geNorm: Tbp, Xbp1, Capon

aTub

0.62

0.50 - 1.02

0.009


GAPDH

0.60

0.32 - 1.06

0.007


MMP

2.64

1.06 - 7.18

0.003


NormFinder I: Tbp, Xbp1

aTub

0.61

0.51 - 0.97

0.001


GAPDH

0.59

0.33 - 0.97

0.002


MMP

2.60

0.90 - 7.68

0.005


NormFinder II: Xbp1, Stx16

aTub

0.71

0.51 - 1.13

0.034


GAPDH

0.69

0.44 - 1.08

0.013


MMP

3.02

1.05 - 9.80

0.003


Classical HKGs: Tbp, aTub, GAPDH

MMP

3.66

1.00 - 13.16

0.003


All genes

MMP

3.27

1.11 - 11.07

0.002


*x-fold change in gene expression in the induced samples compared to the control group.

Spanier et al. BMC Molecular Biology 2010 11:50   doi:10.1186/1471-2199-11-50

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