Table 1

Variation and stability of the rpsO, rpsOΔ and rpsT transcripts as a function of Hfq quantity.

rpsO mRNA (**)

rpsOΔ mRNA (**)

Strains

t1/2 (min) (*)

Relative RNA abundance at T = 0

t1/2 (min) (*)

Relative RNA abundance at T = 0

wt

0.98+/-0.12

100

hfq

1.79+/-0.13

10.3

wt/pGEM3

1.17+/-0.14

100

wt/pTX367

0.98+/-0.16

222

wt/pΔS15AUG

1.13+/-0.1

100

3.16+/-0.07

100

hfq/pΔS15AUG

2.09+/-0.12

50

4.67+/-0.09

62.5

rpsT mRNA

Strains

t1/2 (min) (*)

Relative RNA abundance at T = 0

wt P1-t1

1.31+/-0.17

100

wt P2-t1

1.57+/-0.17

100

hfq P1-t1

2.42+/-0.12

66.7

hfq P2-t1

5.43+/-0.07

16.9


(*) Half-lives of transcripts were measured in strains N3433 (wt) and IBPC929 (hfq), transformed, when indicated, with pGEM3 empty vector, its derivative pTX367 expressing the hfq gene, or with plasmid pΔS15AUG bearing the constitutively expressed truncated rpsO gene.

(**) The rpsO and the rpsOΔ transcripts originated from the chromosome and the pΔS15AUG plasmid, respectively.

Le Derout et al. BMC Molecular Biology 2010 11:17   doi:10.1186/1471-2199-11-17

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