Determination of the optimal number of reference genes calculated by geNorm. Determination of the optimal number of reference genes for accurate normalisation of gene expression. Average pairwise variations Vn/Vn+1 are calculated between the normalisation factors NFn and NFn+1 to indicate whether inclusion of extra reference gene adds to the stability of the normalisation factor.
Maroufi et al. BMC Molecular Biology 2010 11:15 doi:10.1186/1471-2199-11-15