Table 6

Induction of Fluc expression in the presence of Cd

Fold induction

Fluc/Rluc ± SD (% SD)

Fluc/B2M ± SD (% SD)

Fluc/ActB ± SD (% SD)


pMN85

1.34 ± 0.42

1.07 ± 0.27

1.35 ± 0.28

pMN86

61.42 ± 10.12 (16%)

71.38 ± 16.24 (23%)

74.92 ± 25.80 (35%)


The normalized copy number of the Cd-treated samples was compared to the normalized copy number of the untreated samples for pMN85 (PTK-Fluc) and pMN86 (MRE-PTK-Fluc) providing values for the fold induction of the Cd-directed Fluc expression for each of the reference genes (Rluc, B2M and ActB). Abbreviations: SD- standard deviation

Jiwaji et al. BMC Molecular Biology 2010 11:103   doi:10.1186/1471-2199-11-103

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