The effect of an increasing transfection concentration of pRL-SV40 DNA, a control plasmid encoding Rluc, on Rluc gene expression. Rluc expression was normalized to ActB gene expression. Each sample represents the expression of Rluc/ActB in a total RNA concentration of 0.2 μg.
Jiwaji et al. BMC Molecular Biology 2010 11:103 doi:10.1186/1471-2199-11-103