Expression variability of reference genes. Analysis of reference gene expression on TaqMan Low Density Arrays. a) cDNA from a single RT-transcription. b) pooled cDNA samples from normal tissue. The genes POLR2A, TFRC, PPIA and RPLPO had the lowest expression variability. c) cDNA from fresh frozen tissue. RPLPO, PPIA and UBC had the lowest expression variability, TBP was not expressed in the tissue. On the x-axis, the stability values are plotted. Whiskers represent range of data of 4 samples.
Fassunke et al. BMC Molecular Biology 2010 11:100 doi:10.1186/1471-2199-11-100