Research article
RAD51 paralogs promote homology-directed repair at diversifying immunoglobulin V regions
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* Corresponding author: Nancy Maizels maizels@u.washington.edu
1 Department of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195-7650 USA
2 Department of Immunology, University of Washington School of Medicine, Seattle, WA 98195-7650, USA
3 Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA, USA
BMC Molecular Biology 2009, 10:98 doi:10.1186/1471-2199-10-98
Published: 28 October 2009Additional files
Additional file 1:
Vλ sequences in DT40 RAD51D-GFP transfectants. Sequences of 31 diversified Vλ sequences, aligned to the Vλ germ line sequence, with CDRs underlined. Blue, gene conversion tracts; red, nontemplated mutations.
Format: TIFF Size: 2.2MB Download file
Additional file 2:
Vλ sequences in DT40 XRCC2-GFP transfectants. Sequences of 27 diversified Vλ sequences, aligned to the Vλ germ line sequence, with CDRs underlined. Blue, gene conversion tracts; red, nontemplated mutations.
Format: TIFF Size: 1.8MB Download file
Additional file 3:
Conversion tracts in DT40 GFP transfectants. (A) Sequences of 12 diversified Vλ sequences, aligned to the Vλ germ line sequence, with CDRs underlined. Blue, gene conversion tracts; red, nontemplated mutations. (B) Schematic diagram of mutations in 12 Vλ regions from DT40 GFP transfectants, aligned with the germline Vλ region (top line). Notations as in Figure 7A.
Format: TIFF Size: 1.8MB Download file