Additional file 3.

Representative amplification plots and melting curves obtained in the RT-qPCR efficiency test. Four to five ten-fold serial dilutions were plotted against the logarithm of cDNA template concentration. Amplification plots and melting curve images were collected using StepOne software v2.0 (Applied Biosystems).

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Hu et al. BMC Molecular Biology 2009 10:93   doi:10.1186/1471-2199-10-93