Gene expression stability and pairwise variation of the candidate genes as predicted by geNorm. A. Mean expression stability (M) following stepwise exclusion of the least stable gene across all treatment groups. The least stable genes are on the left, and the most stable on the right. B. The optimal number of reference genes required for effective normalization. The pairwise variation (Vn/Vn+1) was analyzed between the normalization factors NFn and NFn+1 by geNorm program to determine the optimal number of reference genes required for RT-qPCR data normalization.
Hu et al. BMC Molecular Biology 2009 10:93 doi:10.1186/1471-2199-10-93