Purity and stoichiometry of Ku heterodimer complexes. A) The Ku70/80ΔC and wild type Ku protein preparations were subject to SDS-PAGE and proteins detected by staining with Coomassie Blue, transferred to PVDF and detected with Ku (p70) Ab-4 or Ku (p80) Ab-7 antibodies as indicated in the figure. B) The C493A and C493S point mutants were also analyzed by SDS-PAGE and western blot analysis.
Bennett et al. BMC Molecular Biology 2009 10:86 doi:10.1186/1471-2199-10-86