Table 1

Methods analysed

Method

Basic chemistry

Physical stress

Temperature stress

Yield (μg/ul)

260/280 ratio

260/230 ratio


PGTX beads

phenol, glycerol, guanidine, triton x

bead beating

N/A

1.70

2.1

1.9


Trizol beads

phenol, guanidine isothiocyanate (proprietary)

bead beating

N/A

1.63

2.0

1.7


PGTX 95

phenol, glycerol, guanidine, triton x

N/A

95°C

1.29

2.1

2.3


Trizol 95

phenol, guanidine isothiocyanate (proprietary)

N/A

95°C

1.03

2.1

1.9


Trizol std

phenol, guanidine isothiocyanate (proprietary)

N/A

N/A

0.47

1.9

1.6


BCP

phenol, chloroform

bead beating

N/A

0.82

1.8

2.2


Description, for each method analysed, of basic chemistry and cell lysis strategy followed. Bead beating and heat were used as described in Methods. (N/A – not applicable).

Pinto et al. BMC Molecular Biology 2009 10:79   doi:10.1186/1471-2199-10-79

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