DNA contamination detection. Agarose gel separation of PCR products. For each of the six described methods, 20 ng of RNA from 4 different RNA extractions were used to perform PCR (using the "ftsZ sense" and "ftsZ antisense" primers). Resulting products were analysed after 25 (A) and 36 (B) cycles of PCR. Lanes M – double stranded DNA molecular weight markers (GeneRuler 100 bp DNA Ladder, Fermentas). To different extents, all extractions showed signs of genomic DNA contamination.
Pinto et al. BMC Molecular Biology 2009 10:79 doi:10.1186/1471-2199-10-79